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    What is the sequence alignment of DHRS1 and HSD11B1?Fig. 1. Sequence alignment of human DHRS1 with two well-known human members of SDR cluster 3 – retinoldehydrogenase 10 (RDH10) and 11β-hydroxysteroid dehydrogenase 1 (HSD11B1). Shaded amino acids are involved in the catalytic mechanism.
    What is the recombinant form of DHRS1?The recombinant form of DHRS1 was purified using the detergent n-dodecyl-β-D-maltoside, and DHRS1 was proven to be an NADPH-dependent reductase that is able to catalyse the reductive conversion of some steroids (estrone, androstene-3,17-dione and cortisone), as well as other endogenous substances and xenobiotics.
    How to determine DHRS1 expression in selected human tissues?Two methods were used to determine the DHRS1 expression in selected human tissues - RT-qPCR for absolute quantification of DHRS1 mRNA and western blotting with specific antibodies for the semi-quantitative detection of DHRS1 protein.
    What are the features of SDR members in DHRS1?Typical features of the SDR members are present in the DHRS1 sequence – the glycine-rich motif (TGxxxGxG) and the catalytically active amino acids (positions S151, Y163, and K167). Fig. 1.
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