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Comparison and validation of Oomycetes metabarcoding primers for
Mar 7, 2019 · The two nested PCR approaches, DC6/ITS7 x Oom18S/ITS7 (PERO_ITS) and Oom18S/ITS7 × 5.8S1R/18ph2F (PHYTO_ITS), proved reliable for qualitative characterisation …
Oomycete-specific ITS primers for identification and …
We developed a novel taxon-specific PCR assay for the ITS region-based identification of oomycetes. When compared with the previously developed ITS-O, ITS6 and ITS7 primers, the …
Degenerate ITS7 primer enhances oomycete community …
Metagenomic analysis of oomycetes through deep amplicon sequencing has been conducted primarily using the ITS6-ITS7 primer set that targets the ITS1 region. While this primer set …
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Oomycete-specific ITS primers for identification and metabarcoding
Aug 31, 2016 · High throughput sequencing of 20 soil samples from forest nurseries and bordering areas, using the primer pair ITS1oo/ITS4, recovered more than 400 oomycete OTUs, which is …
An improved high throughput sequencing method for studying …
Mar 1, 2015 · Our preliminary experiments using ITS6 and ITS4 as primers in a PCR followed by ITS6 and ITS7 in a semi-nested approach showed that an efficient amplification could be …
Degenerate ITS7 primer enhances oomycete community …
Oomycete communities from 35 soil samples collected from asymptomatic and root rot diseased sites in pea fields across Alberta were analyzed simultaneously using ITS6-ITS7 and ITS6 …
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Comparison between the relative abundance of oomycete genera …
While this primer set shows a perfect match to most oomycete taxa, ITS7 contains three mismatches to the corresponding binding site of plant pathogens within the genus Aphanomyces.
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