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  2. FDAA is 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide, also called Marfey's reagent. The compound reacts with primary amines and is used as a derivatization reagent for UV detection in liquid chromatography methods. The reagent provides for detection at 340nm with nanomole sensitivity.

    FDAA (Marfey's Reagent) (1-fluoro-2-4-d…

    www.thermofisher.com/order/catalog/product/48895
    Marfey’s reagent, 1-fluoro-2,4-dinitrophenyl-5+alanine amide reacts (Scheme l), with the optical isomers of amino acids to form diastereomeric N-aryl derivatives which can be separated by conventional HPLC methods.

    (PDF) Application of marfey's reagent in …

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    What is marfey's reagent used for?This article describes some of the uses of Marfey's reagent, 1-fluoro-2,4-dinitrophenyl-5-L-alanine amide (FDAA), a pre-column derivatizing reagent for the separation of enantiomeric isomers of amino acids and amine compounds. An introduction to the basic implementation of this reagent and its historical development are presented.

    Marfey's reagent: Past, present, and future uses of 1‐fluoro‐2,4

    analyticalsciencejournals.onlinelibrary.wiley.com
    Can marfey's reagent be used for enantioseparation of amino groups?The present paper assesses and reviews applications of Marfey’s reagent and its chiral variants (i.e. other FDNP reagents) for enantioseparation of certain amino group containing drugs/amino acids, and to provide some case studies on enantiomeric separations that are important for the pharmaceutical industry.

    Assessment and application of Marfey’s reagent and analogs

    analyticalsciencejournals.onlinelibrary.wiley.com
    What reagents were used in marfey's test?Marfey’s reagent, 1-fluoro-2,4-dinitrophenyl-5-L-alanine amide (L-FDAA), amino acid standards, formic acid, trifluoroacetic acid and ammonium chloride were purchased from Sigma-Aldrich. HPLC grade solvents (water and acetonitrile) and analytical grade acetone were from Merck. Acetonitrile-d 3 99.80% D was purchased from Eurisotop.

    Expanding the utility of Marfey’s analysis by using HPLC ... - Springer

    link.springer.com
    Can marfey's reagent be used for chromatographic resolution of D- and L-amino acids?Marfey’s reagent has proven to be an effective and commonly used chiral derivatizing agent for the chromatographic resolution of d - and l - amino acids [ 24, 31, 32 ]. Despite its popularity, a systematic study of its application for the resolution and quantification of all 20 common dl -amino acids has not been reported to our knowledge.

    LC-MS/MS-Based Separation and Quantification of Marfey’s ... - Springer

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  4. PubMed
    https://pubmed.ncbi.nlm.nih.gov/15503232

    Marfey's reagent for chiral amino acid analysis: a review

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  5. Thermo Fisher Scientific
    https://www.thermofisher.com/order/ca…

    FDAA (Marfey's Reagent) (1-fluoro-2-4-dinitrophenyl-5 …

    WEBThermo Scientific Pierce Marfey's Reagent (FDAA) reacts with primary amines to enable the quick and easy separation and quantitation of optical isomers of amino acids by reverse-phase chromatography. FDAA is 1 …

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    Marfey’s reagent for chiral amino acid analysis: A review
    uni-giessen.de
    Marfey's reagent | Sigma-Aldrich
    sigmaaldrich.com
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  6. Studies of marfey's reagent
    ScienceDirect
    Review Use of Marfey's reagent and analogs for chiral amino acid analysis: Assessment and applications to natural products and biological systems☆
    The present paper describes an updated knowledge and status on Marfey's reagent (MR), 1-fluoro-2,4-dinitrophenyl-5-l-alanine amide (FDNP-l-Ala-NH2). The reagent is used for pre-column derivatization of amino acids followed by HPLC separation of the
    Published: 2011/11/01
    Cited By: 0
    Keywords: Ac acetyl; A…
    analyticalsciencejournals.onlinelibrary.wiley.com
    Assessment and application of Marfey’s reagent and analogs in enantioseparation: a decade’s perspective
    Of the various methods available for high-performance liquid chromatography separation of enantiomers (of e.g. amino acids and amino group containing compounds) by the pre-column derivatization approach, use of Marfey’s reagent has been most successful
    Author: Sonika Sethi, J…
    Published: 22 Septem…
    Cited By: 14
    analyticalsciencejournals.onlinelibrary.wiley.com
    Marfey's reagent: Past, present, and future uses of 1-fluoro-2,4-dinitrophenyl-5-L-alanine amide
    This article describes some of the uses of Marfey's reagent, 1-fluoro-2,4-dinitrophenyl-5-L-alanine amide (FDAA), a pre-column derivatizing reagent for the separation of enantiomeric isomers of amino acids and amine compounds. An introduction to the
    Author: Clayton B'Hym…
    Published: 28 Januar…
    Cited By: 85
    National Center for Biotechnology Information
    LC-MS/MS-Based Separation and Quantification of Marfey’s Reagent Derivatized Proteinogenic Amino Acid DL-Stereoisomers
    There are 20 proteinogenic amino acids (AAs), which are incorporated as their L-enantiomers (except for glycine, which is achiral) in proteins. Their corresponding D-enantiomers also play important roles in many biological systems and processes [1]. The
    Author: Navid J. Ayon…
    Published: 2019/03
    DOI: 10.1007/s13361-0…
    PubMed
    Assessment and application of Marfey's reagent and analogs in enantioseparation: a decade's perspective
    Of the various methods available for high-performance liquid chromatography separation of enantiomers (of e.g. amino acids and amino group containing compounds) by the pre-column derivatization approach, use of Marfey's reagent has been most successful
    Author: Sonika Sethi
    Published: 2021/1
    Keywords: Marfey’s re…
    PubMed
    Use of Marfey's reagent and analogs for chiral amino acid analysis: assessment and applications to natural products and biological systems
    The present paper describes an updated knowledge and status on Marfey's reagent (MR), 1-fluoro-2,4-dinitrophenyl-5-L-alanine amide (FDNP-L-Ala-NH(2)). The reagent is used for pre-column derivatization of amino acids followed by HPLC separation of
    Author: R Bhushan
    Published: 2011/11/01
    Keywords: National In…
    ScienceDirect
    Application of the advanced Marfey’s method for the determination of the absolute configuration of ogipeptins
    Ogipeptins A–D are cyclic lipopeptides that were isolated from culture broth by Daiichi Sankyo. Each of them showed antimicrobial activity against Gram-negative bacteria. Ogipeptins are composed of seven amino acid residues, and three of them are rare
    Published: 2022/04/27
    Cited By: 0
    Keywords: Ogipeptin; …
    National Center for Biotechnology Information
    Discovery and Biocatalytic Application of a PLP-Dependent Amino Acid γ-Substitution Enzyme that Catalyzes C-C Bond Formation
    Nature is remarkable in building and using structurally diverse amino acids.1–2 Nonproteinogeneic amino acids (NAAs), which constitute 96% of the naturally occurring amino acids,1 are frequently incorporated into small molecules to broaden reactivity
    Author: Mengbin Chen…
    Published: 2020/06/06
    DOI: 10.1021/jacs.0c03…
    ScienceDirect
    Review Use of Marfey's reagent and analogs for chiral amino acid analysis: Assessment and applications to natural products and biological systems☆
    The present paper describes an updated knowledge and status on Marfey's reagent (MR), 1-fluoro-2,4-dinitrophenyl-5-l-alanine amide (FDNP-l-Ala-NH2). The reagent is used for pre-column derivatization of amino acids followed by HPLC separation of the
    Published: 2011/11/01
    Cited By: 105
    Page Count: 14
  7. ScienceDirect
    https://www.sciencedirect.com/science/article/pii/S1570023211003989

    Use of Marfey's reagent and analogs for chiral amino acid …

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    Marfey’s reagent for chiral amino acid analysis: A review
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    Synthesis and application of new chiral variants of Marfey ... - …
    akjournals.com
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  8. Analytical Science Journals
    https://analyticalsciencejournals.onlinelibrary.wiley.com/...

    Assessment and application of Marfey’s reagent and …

    WEBSep 22, 2020 · The present paper assesses and reviews applications of Marfey’s reagent and its chiral variants (i.e. other FDNP reagents) for enantioseparation of certain amino group containing drugs/amino acids, …

    Tags:
    Amino Acids
    Marfey's Reagent Derivatization
    Publish Year:2021
  9. Justus-Liebig-Universität Gießen
    https://www.uni-giessen.de/.../prof/lmw/pdf-brueckner/marfeys-reagent
    [PDF]

    Marfey’s reagent for chiral amino acid analysis: A review

    Tags:
    Chiral Amino Acid Analysis
    Reagent
  10. Analytical Science Journals
    https://analyticalsciencejournals.onlinelibrary.wiley.com/...

    Marfey's reagent: Past, present, and future uses of …

    WEBJan 28, 2003 · This article describes some of the uses of Marfey's reagent, 1-fluoro-2,4-dinitrophenyl-5-L-alanine amide (FDAA), a pre-column derivatizing reagent for the separation of enantiomeric isomers of amino …

    Tags:
    Amino Acids
    Marfey's Reagent Derivatization
    Marfey's Reagent Sigma
  11. National Center for Biotechnology Information
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6417927

    LC-MS/MS-Based Separation and Quantification of Marfey’s …

    Tags:
    Amino Acids Derivatization Lc Ms
    Publish Year:2019
    Published:2019/03
  12. Springer
    https://link.springer.com/article/10.1007…

    LC-MS/MS-Based Separation and Quantification of …

    WEBNov 12, 2018 · In this study, we investigated the use of Marfey’s reagent (chiral) derivatization coupled with LC-MS/MS-based separation and detection of the resulting diastereomers for quantification of the 19 …

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    Publish Year:2019
    Navid J. Ayon, Amar Deep Sharma, William G. Gutheil
  13. PubMed
    https://pubmed.ncbi.nlm.nih.gov/32959901

    Assessment and application of Marfey's reagent and analogs in …

    Tags:
    Amino Acids
    Marfey's Reagent Derivatization
    Assessment Reagent
  14. thermofisher.com
    https://assets.thermofisher.com/TFS-Assets/LSG/...
    [PDF]

    INSTRUCTIONS FDAA, Marfey’s Reagent - Thermo Fisher …

  15. Springer
    https://link.springer.com/article/10.1007/s00726-004-0118-0

    Marfey’s reagent for chiral amino acid analysis: A review

    Tags:
    Chiral Amino Acid Analysis
    R Bhushan, H Brückner
    Publish Year:2004
  16. Springer
    https://link.springer.com/article/10.1007/s00216-022-04339-2

    Expanding the utility of Marfey’s analysis by using HPLC

    Tags:
    Amino Acids
    Reagent
  17. PubMed
    https://pubmed.ncbi.nlm.nih.gov/21737363

    Use of Marfey's reagent and analogs for chiral amino acid …

    Tags:
    Amino Acids
    Marfey's Reagent Derivatization
    Chiral Amino Acid Analysis
  18. MilliporeSigma
    https://www.sigmaaldrich.com/US/en/search/marfey's...

    Marfey's reagent | Sigma-Aldrich - MilliporeSigma

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    Reagent
    Marfey
  19. PubChem
    https://pubchem.ncbi.nlm.nih.gov/compound/Marfey_s-reagent

    Marfey's reagent | C9H9FN4O5 | CID 5486955 - PubChem

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    Marfey's Reagent Derivatization
    Marfey's Reagent Sigma
  20. ScienceDirect
    https://www.sciencedirect.com/science/article/pii/S0040403922001575

    Application of the advanced Marfey’s method for the …

  21. ACS Publications
    https://pubs.acs.org/doi/10.1007/s13361-018-2093-9

    LC-MS/MS-Based Separation and Quantification of Marfey’s …

    Tags:
    Amino Acids Derivatization Lc Ms
    19 Amino Acid
    Lc Ms Amino Acid Analysis
  22. Nature
    https://www.nature.com/articles/s41589-024-01619-z

    The β-subunit of tryptophan synthase is a latent tyrosine synthase

  23. PubMed
    https://www.semanticscholar.org/paper/Marfey’s...

    Marfey’s reagent for chiral amino acid analysis: A review

    Tags:
    Chiral Amino Acid Analysis
    Reagent
  24. National Center for Biotechnology Information
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7335459

    Discovery and Biocatalytic Application of a PLP-Dependent …

    Tags:
    Amino Acid
    Publish Year:2020
    10.1021/jacs.0c03535
    Published:2020/06/06
  25. ScienceDirect
    https://www.sciencedirect.com/science/article/abs/...

    Use of Marfey's reagent and analogs for chiral amino acid …

    Tags:
    Amino Acids
    Marfey's Reagent Derivatization
    Chiral Amino Acid Analysis
  26. Taylor & Francis Online
    https://www.tandfonline.com/doi/pdf/10.1271/bbb.70371
    [PDF]

    Relative and Absolute Configuration of Antitumor Agent SW …

    Tags:
    Amino Acid
    Antitumor Agent SW-163D
  27. plos.org
    https://journals.plos.org/plosone/article?id=10...

    Phenotypic and Genotypic Characterization of Daptomycin

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    Marfey's Reagent Derivatization
    Marfey's Reagent Sigma
    Publish Year:2014