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- Marfey's reagent, also known as FDAA, is a derivatization reagent for UV detection in liquid chromatography methods1. It is 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide1. The compound reacts with primary amines1. The reagent provides for detection at 340nm with nanomole sensitivity1. Marfey's reagent reacts with the optical isomers of amino acids to form diastereomeric N-aryl derivatives which can be separated by conventional HPLC methods2.Learn more:✕This summary was generated using AI based on multiple online sources. To view the original source information, use the "Learn more" links.FDAA is 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide, also called Marfey's reagent. The compound reacts with primary amines and is used as a derivatization reagent for UV detection in liquid chromatography methods. The reagent provides for detection at 340nm with nanomole sensitivity.www.thermofisher.com/order/catalog/product/48895Marfey’s reagent, 1-fluoro-2,4-dinitrophenyl-5+alanine amide reacts (Scheme l), with the optical isomers of amino acids to form diastereomeric N-aryl derivatives which can be separated by conventional HPLC methods.www.academia.edu/14985990/Application_of_marf…
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WebSeparation mechanisms of resolution of amino acid diastereomers and replacement of Ala-NH2 by suitable chiral moieties providing structural analogs and different chiral variants and their application as a derivatizing agent to examine the efficiency, and reactivity of the …
- Author: R Bhushan, H Brückner
- Publish Year: 2004
See results only from pubmed.ncbi.nlm.nih.govLC-MS/MS-Based Separatio…
In this study, we investigated the use of Marfey’s reagent (chiral) derivatization …
Marfey's reagent | C9H9FN4…
Marfey's reagent | C9H9FN4O5 | CID 5486955 - structure, chemical names, …
Use of Marfey's reagent an…
PMID: 21737363. DOI: 10.1016/j.jchromb.2011.05.058. Abstract. …
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Abstract. Of the various methods available for high-performance liquid …
WebFDAA is a derivatization reagent for UV detection of amino acids in liquid chromatography. It reacts with primary amines and enables chiral separation of D- and …
WebMarfey was the first to synthesise 1-fluoro-2,4-dinitrophe-nyl-5-L-alanineamide (FDAA) in high yield (76%) by the reaction of 1,5-difluoro-2,4-dinitrobenzene (DFDNB) and L …
WebNov 1, 2011 · The present paper describes an updated knowledge and status on Marfey's reagent (MR), 1-fluoro-2,4-dinitrophenyl-5-l-alanine amide (FDNP-l-Ala-NH 2). The …
Assessment and application of Marfey’s reagent and …
WebSep 22, 2020 · Assessment and application of Marfey’s reagent and analogs in enantioseparation: a decade’s perspective - Sethi - 2021 - Biomedical Chromatography - Wiley Online Library.
WebOct 22, 2004 · Keywords: Marfey’s reagent (1-Fluoro-2,4-dinitrophenyl-5- L -alanine amide, FDAA) – Pre-column derivatization – Enantiomeric resolution – Amino acids, …
Marfey's reagent: Past, present, and future uses of …
WebJan 28, 2003 · Marfey's reagent: Past, present, and future uses of 1‐fluoro‐2,4‐dinitrophenyl‐5‐L‐alanine amide - B'Hymer - 2003 - Journal of Separation Science - Wiley Online Library.
WebMarfey’s reagent for chiral amino acid analysis: A review | Semantic Scholar. DOI: 10.1007/s00726-004-0118-0. Corpus ID: 10014038. Marfey’s reagent for chiral amino …
LC-MS/MS-Based Separation and Quantification of …
WebNov 12, 2018 · In this study, we investigated the use of Marfey’s reagent (chiral) derivatization coupled with LC-MS/MS-based separation and detection of the resulting diastereomers for quantification of the 19 …
Expanding the utility of Marfey’s analysis by using HPLC
WebOct 4, 2022 · Marfey’s reagent (MR) incorporates a strong chromophore in its structure that facilitates chromatographic detection of the derivatized amino acids by their characteristic …
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WebMay 14, 2024 · Putative O-alkylation product of the Tyr and Marfey’s reagent, with the same mass as the desired N-alkylation product, was seen as an early single peak. A similar O …
Application of the advanced Marfey’s method for the …
WebApr 27, 2022 · The advanced Marfey’s method was applied to determine the absolute configuration of β-OH Dab in ogipeptin A. Herein, we describe the determination method …
WebFDAA, Marfey’s Reagent. 48895. MAN0016377. Rev. A.0 Pub. Part No. 2161295.3. Number. 48895. Description. FDAA, Marfey’s Reagent (1-fluoro-2-4-dinitrophenyl-5-L …
Marfey's reagent | Sigma-Aldrich - MilliporeSigma
WebSynonym (s): L-AP-4, L-AP4. Empirical Formula (Hill Notation): C4H10NO5P. CAS No.: 23052-81-5. Molecular Weight: 183.10. A7929. optical purity optical purity: ≥95% (HPLC, …
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Use of Marfey's reagent and analogs for chiral amino acid …
WebNov 1, 2011 · The present paper describes an updated knowledge and status on Marfey's reagent (MR), 1-fluoro-2,4-dinitrophenyl-5- l -alanine amide (FDNP- l -Ala-NH 2). The …
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