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IHC staining protocol for whole mount samples | Abcam
Protocol and troubleshooting tips for whole mount immunohistochemical staining. Whole mount staining is the staining of small pieces of tissue – usually embryos – without sectioning. Whole mount staining is very similar to immunocytochemistry (ICC) or staining of cryosections. See more
Some researchers view and obtain images of embryos as they are. The whole embryo can be imaged while floating in glycerol buffer in a petri dish, before … See more
As the embryo grows, it will become too large to stain. The various reagents, including fixative, antibody and developing solution will not be able to permeate … See more
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IHC staining protocol for whole mount samples | abcam
WEBDetailed IHC staining protocol for whole mount samples of chick, mouse, Drosophila or zebrafish embryos including troubleshooting tips.
Protocol Guide: Immunofluorescent Staining of Whole-Mount …
WEBWhole-mount staining of organoids can be used for antibody based characterization. In this organoid staining protocol we detail methods to fix, permeabilize, and stain whole …
- Studies of whole mount tissue staining
Versatile whole-organ/body staining and imaging …
WEBApr 27, 2020 · By using CUBIC-HistoVIsion for 3D staining, tissue clearing, and volumetric imaging, we successfully stained and imaged whole adult mouse brains, a whole adult marmoset brain hemisphere,...
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Whole mount - Abcam
WEBWhole mount fluorescent immunohistochemistry. The advantage of using fluorescence to stain whole mount sections is that confocal microscopy can be used to section through …
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Whole-Mount Skeletal Staining - PMC - National Center for …
WEBWhole-mount skeletal staining permits evaluation of the shapes and sizes of skeletal elements in their appropriate locations. The technique is thus the major method for …
Protocol for whole-mount X-gal staining combined with tissue …
WEBMar 3, 2022 · Here we describe an optimized protocol for X-gal staining of tissue clearing embryo and adult mouse using CUBIC. The activity of LacZ knock-in reflecting …
Whole-Mount Immunofluorescence Staining of Early Mouse …
WEBSep 19, 2020 · Immunofluorescence staining enables the visualization of protein expression at a cellular or even sub-nuclear level. Whole-mount staining preserves the …
Protocol: an improved and universal procedure for whole-mount ...
WEBOct 28, 2015 · In plants robust and reliable techniques are highly required for the accurate whole-mount visualization of subcellular protein localization in relatively thick …
Whole-Mount Immunofluorescence Staining, Confocal …
WEBDec 22, 2020 · Whole-mount immunofluorescence staining is intended for smaller sections of tissue without the need for manual sectioning. To this purpose, the mouse heart is dissected, with unwanted tissue removed, …
WEBFor whole mount and confocal imaging, the tissue needs to be thin or deformable, such as the diaphragm, spinotrapezius, or fat. The tissue can be stored for a few days in the …
Whole-mount three-dimensional imaging of internally localized ...
WEBFeb 9, 2012 · In this protocol we describe the whole-mount fixation and multimarker staining procedure, the tissue transparency treatment, microscopy and the analysis of …
Whole-mount immunofluorescence staining of blood and …
WEBJun 6, 2023 · Here, we present a protocol for whole-mount immunofluorescence staining of blood and lymphatic vessels in the adult mouse small intestine. We describe the steps …
A clearing-free protocol for imaging intact whole adipose tissue ...
WEBMar 18, 2022 · Whole mount adipose tissue processing for immunofluorescence staining and imaging. •. Clearing-free protocol that uses Z-depth reduction and autofluorescence …
Fluorescence-based Single-cell Analysis of Whole-mount-stained …
WEBJun 20, 2021 · Here, we present a simple protocol for combined whole-mount immunostaining, tissue-clearing, and optical analysis of large-scale (approx. 1 mm) 3D …
iDISCO: a simple, rapid method to immunolabel large tissue
WEBNov 6, 2014 · We describe a simple, rapid, and inexpensive method, iDISCO, that permits whole-mount immunolabeling with volume imaging of large cleared samples ranging …
Immunohistochemistry of Whole-Mount Mouse Embryos - CSH …
WEBImmunohistochemistry of Whole-Mount Mouse Embryos. Alex Joyner and. Nancy Wall. This protocol was adapted from “Techniques for Visualizing Gene Products, Cells, …
Whole-Mount Immunofluorescence Staining, Confocal Imaging …
WEBDec 22, 2020 · Whole-mount immunofluorescence staining is intended for smaller sections of tissue without the need for manual sectioning. To this purpose, the mouse …
Mouse Mammary Gland Whole Mount Preparation and Analysis
WEBJul 7, 2018 · This protocol describes mammary gland isolation, whole mount preparation and analysis. Mammary gland tissue, which is removed postmortem, is stained with …
Whole-Mount Imaging of Adipose Tissue Macrophages - PubMed
WEBWhole-amount staining is a powerful technique for macrophage characterization within the 3D environment of the adipose tissue, enabling the visualization of different …
Methods in Enzymology (MIE): Methods of Adipose Tissue Biology-
WEBIn this chapter, we will provide detailed protocols for preparing and imaging whole mount, paraffin sectioned and frozen sectioned adipose tissue. We will also provide discussion …
Cytology Stains - MilliporeSigma
WEBCytology Stains. Cytology is the of analysis of exfoliated cells and cells removed from tissues of the human body allowing for the rapid diagnosis of many common diseases. …
Visualization of 3D White Adipose Tissue Structure Using Whole …
WEBNov 17, 2018 · Here we present whole-mount staining, an immunohistochemistry method that preserves intact adipose tissue morphology with minimal processing steps. Hence, …
Immunostaining of Whole-Mount Retinas with the CLARITY …
WEBMar 6, 2021 · Here, we adapted the CLARITY method for whole-mount mouse retinas by polymerizing them with an acrylamide monomer to form a nanoporous hydrogel and …
In Situ Immunofluorescence Imaging of Vital Human Pancreatic …
WEBJun 6, 2024 · The combination of in situ immunofluorescence staining and fiber-optic microscopy visualizes structures in vital tissues and could be proposed as an useful tool …
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