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  2. There are two major techniques for preparation of histologic sections12:
    • Permanent sections: This technique gives the best quality of specimen for examination, at the expense of time. The fresh specimen is immersed in a fluid called a fixative for several hours (the necessary time dependent on the size of the specimen).
    • Frozen sections: A pathologist prepares the biopsied tissue for examination via a "frozen section," (a procedure that takes about 30 minutes), and a "permanent section," (a procedure that takes 2 to 3 days). In brief, the frozen section is a quick way of determining whether or not cancer is present in the tissue. The permanent section is the most accurate method.
    Fresh tissue samples must be preserved for future examination3. This process is called fixation, and the resulting specimen is described as fixed. Boiling an egg and pickling a cucumber represent examples of fixation, in which heat or chemistry stabilizes the organic materials.
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    There are two major techniques for preparation of histologic sections: Permanent sections This technique gives the best quality of specimen for examination, at the expense of time. The fresh specimen is immersed in a fluid called a fixative for several hours (the necessary time dependent on the size of the specimen).
    www.training.seer.cancer.gov/treatment/surgery/all…
    A pathologist prepares the biopsied tissue for examination via a "frozen section," (a procedure that takes about 30 minutes), and a "permanent section," (a procedure that takes 2 to 3 days). In brief, the frozen section is a quick way of determining whether or not cancer is present in the tissue. The permanent section is the most accurate method.
    www.vabreastcenter.com/ermanent-frozen-sections
    Fresh tissue samples must be preserved for future examination. This process is called fixation, and the resulting specimen is described as fixed. Boiling an egg and pickling a cucumber represent examples of fixation, in which heat or chemistry stabilizes the organic materials.
    histology.siu.edu/intro/tissprep.htm
     
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    What specimens should be submitted 'fresh' without fixative?Any specimen requiring an intraoperative consultation (or frozen section), ancillary studies (e.g. flow cytometry, chromosome analysis and/or special send out testing), and muscle biopsies should be submitted "fresh" without fixative.
    When should a specimen be fixed?To prevent degeneration or drying out, the specimen should be fixed as soon as possible. Fixation is a crucial step in preparing specimens for microscopic examination. Its objective is to prevent decay and preserve cells and tissues in a “life-like” state.
    How to prevent autolysis of fresh samples?To prevent autolysis of fresh samples (without fixative) or drying out too quickly, it is important to keep them cold (2°C to 6°C). It is usually best to place a fresh specimen directly in the specimen container and keep cool in the refrigerator as soon as it is collected. Exceptions are noted below.
    What happens when a specimen is accepted in a laboratory?Specimens are usually received in fixative (preservative) but sometimes arrive fresh and must be immediately fixed. Before specimens are accepted by a laboratory, the identification (labeling) and accompanying documentation will be carefully checked, all details recorded, and “specimen tracking” commenced.
     
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    WebOct 1, 2019 · Difference Between a Frozen and Permanent Section. A frozen section is one of two methods for examining tissue samples. The other type of tissue sample is a permanent section. For a permanent

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    WebMay 20, 2021 · Specimens should be sent fresh without delay, specifically labeled “for lymphoma protocol.” Larger specimens and excisions can be immersed in saline solution, whereas cores should be placed on filter …

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